Phis2 酵母单杂
Webb产品名称：Y187-pHis2 Yeast One-Hybrid Media Kit Y187-pHis2 酵母单杂培养基套装（ZC1902） 产品介绍 说明书下载 产品介绍： 酵母杂交培养基套装包含酵母杂交文库筛 … Webb本文针对酵母单杂技术的基本原理、基础知识、主要用途做了简要介绍；并以Y1Hgold（pAbAi-bait）和Y187（pHis2-bait）为例，介绍了自激活检测的目的以及主要 …
Phis2 酵母单杂
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http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html
Webb1、pHis2-Bait或pAbAi-Bait和AD-prey质粒 2、或者Bait和Prey序列，质粒由我们构建 1、提供详细的、完整的实验报告，清晰的实验图片 2、在受体菌的阳性菌 自激活和毒性验证: … Webb1 juni 2024 · Clone the products of the PCR reactions carried out in step 3 into the pHIS2 T-vector obtained in step 5. The T-A ligation system includes 0.2 μg of T-vector of pHIS2, 0.5 μL of PCR products (Y1 + Y3 and Y2 + Y3), 1 μL of 10× ligation buffer, 3 U of T4 ligase, and PEG6000 at final concentration of 10% (w/v).
http://muchong.com/t-9775583-1-pid-2 Webb酵母单杂交技术就是依据这个原理，通过构建可与转录激活结构域结合的蛋白，然后与特异的DNA序列相结合，进而启动下游报告基因的表达。 目前酵母单杂交体系已被广泛应用于验证DNA与蛋白质的相互作用，寻找与目的DNA片段相互作用的蛋白质分子，由此找到了许多新的转录因子。 酵母单杂交技术的优势 ①酵母单杂交技术简单易行，在这个过程中，可 …
Webb产品名称：pHIS2酵母单杂交质粒. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was …
Webb1 mars 2024 · VvACTIN or AtACTIN2 was used as internal references for grape and Arabidopsis. The qRT-PCR primers are listed in Table 1. Table 1. Gene-specific primers used for quantitative real-time PCR assays. 2.7. Transformation of Arabidopsis The 35S: VvWRKY30 vector was created by cloning genes into the pSuper1300 binary vector with … rugworld glasgowWebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog #K1617-1 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ... scarlett brightly ever after creamWebb12 apr. 2024 · 目的：筛选与启动子pHIS2-A互作的蛋白。 方法：以启动子A构建到酵母单杂交启动子载体pHIS2，文库质粒为pGADT7-中山杉文库。 材料： 1．启动子克隆：pHIS2-A。 根据实验需求进行扩增并酶切构建载体，两端的EcoRI和 SacI酶切位点，序列如下： GAATTCAGTTGGAATCACATCTATCATCAGAATGAAAGACCTAAGAAAAAAATGGCA … scarlett brightonWebb1 apr. 2024 · The Y187 strain was prepared for the Y1H assay, we mixed pAD-CmBBX7 and pAD-GUS with pHIS2-CmFTL1pro respectively, and transformed them into Y187 strain using the lithium acetate method.Then inoculated on SD/His-Trp-Leu-plates, and screened for the lowest concentration range of 3-AT that was sufficient to inhibit the growth of … scarlett built homesWebb22 juli 2013 · 酵母单杂交系统 Clontech pHis2说明书 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit （#K1617-1）. scarlett buchananhttp://www.zoonbio.com/molecular/yeast-one-hybrid.html scarlett brightly essence tonerWebb还有一点，我看了pHIS2的序列，在HIS3前面，Pmin区域里也有我的转录因子结合位点，会不会是我的转录因子结合到那里去了？不知道有没有这个可能性啊？这个minimum … scarlett burke commercial